T cell-mediated immunity against infectious diseases 3. Shared idiotopes between anti-class II monoclonal antibodies and major histocompatibility complex restriction site of T cells. The development of malaria vaccine: Induction of T cells is the main stream of sporozoite vaccine develoment.
My interest is in combining immunology with bioengineering to understand how our bodies deal with the invisible but rich microbiological world in which we are immersed.
I'm also a co-founder of Day Zero Diagnosticsan early start-up that's developing a diagnostic for rapidly identifying the species and antibiotic susceptibility of bacterial infections using whole genome sequencing and machine learning.
In my free time I love to travel, take photographs, and spend time with my wonderful husbanddaughter, and friends.
Thesis cytokine cd4 a detailed list of my experiences, please see my CV. For a quick overview of selected research experience, see below. For my PhD, I sought to determine how 1 genital bacteria and 2 injectable hormonal contraceptives e.
Depo-Provera and Nur-isterate modulate host inflammatory responses and HIV acquisition risk in women. The existing paradigm is that vaginal monocolonization by Lactobacillus is normal, and encroachment by other bacteria is pathologic. By characterizing cervicovaginal bacterial communities in a cohort of young South African women using 16S rRNA and shotgun sequencing, we found that the majority of participants had low Lactobacillus abundance and high ecological diversity.
One diverse Prevotella-containing community type strongly correlated with increased female genital inflammation and a 4x higher HIV aquisition rate compared to women with Lactobacillus crispatus dominance. Members of the Prevotella-containing bacterial community induced genital epithelial cells and antigen presenting cells to secrete the same cytokines that we found in vivo, partially through LPS sensing via TLR4.
Our results identify specific bacterial species that not only alter the inflammatory state of the FGT but more broadly impact a woman's risk of acquiring HIV, and we raise the hope of novel preventative therapeutics.
We also investigated the immunological effects of IPCs, the most common form of birth control in sub-Saharan Africa.
Although highly effective as a contraceptive, IPCs are controversially associated with increased HIV susceptibility by an unclear mechanism. We found that IPC users had a 3-fold higher risk of acquiring HIV than women not using a long-term family planning method.
Phenotypic cellular analysis revealed that IPC users also had a significantly higher frequency of activated HIV target cells in the cervix. Since the availability of target cells in the genital mucosa enables early viral replication, recruitment or retention of these cells, the association between IPC use and target cell availability may explain the observed increased HIV acquisition rates in these women.
Furthermore, IPC use was not associated with differences in genital cytokine levels, indicating that cervicovaginal bacteria and exogenous progesterone increase HIV susceptibility by unique pathways. As part of my rotation project, I made a small contribution to the elucidation of a new mechanism of T cell exhaustion and dysfunction in the gut of HIV-infected people by determining the cellular subsets expressing E-cadherin in the lamina propria.AMPs are generally considered as positively charged small peptides, comprising about 12 −50 amino acids,,.The availability of the positively-charged amino acids, such as lysine and arginine, confers a general positive net charge to the AMPs.
cytokine responses and memory differentiation Master’s thesis in Molecular Medicine June Faculty of Medicine Department of Cancer Research and Molecular Medicine Figure CD4+ T cell cytokine production from healthy individuals in response to antigens.
Oxford University, Weatherall Institute of Molecular Medicine, Jackson Lab Masters thesis research: Investigated the mechanisms of neutrophil trafficking in lymphatic vessels, and examined the role of lymphatic endothelial cells in innate immunity.
Topics in CD11b: The innate immune response Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.
thesis, purified CD4+ cells were cultured in well flat-bottomed Stimulation of cytokine synthesis. From the Division of Hematology, Third Department of Medicine. Sutherlandia frutescens / microphylla, an indigenous Southern African shrub commonly known as "cancer bush / kankerbos" has a long traditional African and settler folklore medicinal history, but has recently been hyped by a band of ethno-pirates (those exploiting the traditional culture and intellectual property of indigenous people for financial gain) as a treatment for a ridiculously long.